a project to join

1. Isolation and culture of prokaryotic cells (bacteria and archaea) by agar spreading methods (culture-dependent methods)
2. Phenotypic and chemotaxonomic characterization of halophilic strains
3. Molecular characterization of halophilic strains (ARNr16S)
4. Culture independent methods to study the biodiversity and the dynamic of prokaryotic communities in saline and hypersaline waters (DGGE, FISH...)
5. Culture of bacterial and archeal strains at different scales (in flasks and fermenters) for biomolecules production (batch, feed-batch, continuous cultures)
6. Purification and structural characterization of novel biomolecules ,

Current lines of investigation: ectoine and pigments production at pilot scale by a local collection of halophilic strains, purification and use in the cosmetic industry to manufacture new anti-ageing skincare products
Last publications:
1. Ayadi et al. (2020). The saltern derived Paludifilum halophilum DSM 102817T is a new high yield ectoines producer in minimal medium and under salt stress conditions. 3 Biotech 10 :533 https://doi.org/10.1007/s13205-020-02512-x
2. Frikha et al. (2021) Genome analysis of the salt‑resistant Paludifilum halophilum DSM 102817T reveals genes involved in flux‑tuning of ectoines and unexplored bioactive secondary metabolites.World Journal of Microbiology and Biotechnology https://doi.org/10.1007/s11274-021-03147-7

PA4 - Blue Bioresources

We're looking to joint a project on the development of marine bioresources through fermentor culture, production and purification of novel biomolecules of cosmetic interest. We want to scale up our production of ectoine, an extremolyte with high moisturising power, from laboratory to pilot scale.

halophilic bacteria, fermentation, feed-batch, ectoine, pigments antioxydant, antiage products

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